Detection of SARS-CoV-2 in wastewater in Halifax, Nova Scotia, Canada, using four RT-qPCR assays

نویسندگان

چکیده

Wastewater-based surveillance methods have been implemented in several countries as a tool for monitoring SARS-CoV-2 at community scale. A variety of used concentrating, extracting, and detecting the virus, with no clear consensus on most effective approach. In this note, we report preliminary findings from study that is tracking wastewater Halifax, Nova Scotia, specific focus use four reverse transcriptase quantitative PCR (RT-qPCR) assays virus wastewater. We were able to detect samples during initial rise cases Halifax region early November 2020. Levels targeted gene fragments increased fell response reported COVID-19. The CDC N1 E RT-qPCR demonstrated greater relative sensitivity than N2 N3 detection raw sewage samples.

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ژورنال

عنوان ژورنال: Facets

سال: 2021

ISSN: ['2371-1671']

DOI: https://doi.org/10.1139/facets-2021-0026